CD3E Mouse Monoclonal Antibody [Clone ID: CRIS-7]

CAT#: AM26052BT-N

CD3E mouse monoclonal antibody, clone CRIS-7, Biotin

Conjugation: Unconjugated Biotin


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Specifications

Product Data
Clone Name CRIS-7
Applications FC, IHC
Recommended Dilution Immunohistochemistry on frozen sections: 0.5mg/ml (1:400).
Suggested positive control: Human tonsil.
Has been described to work in FACS.
Does not react in Immunohistochemistry on paraffin fixes cells (epitope is not fixation resistant).
Reactivities Human, Monkey, Rhesus Monkey
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Stimulated human leukocytes
Specificity This antibody detects CD3 antigen on mature human T-cells.
Formulation Phosphate buffered saline pH 7.2 (PBS)
Label: Biotin
State: Lyophilized Ig fraction
Stabilizer: 5 mg/ml bovine serum albumin (BSA)
Preservative: 0.05% (v/v) Kathon CG
Reconstitution Method Reconstitute by adding 0.5ml distilled water.
Concentration 0.2mg/ml (reconstituted)
Purification Affinity purified
Conjugation Biotin
Storage Store lyophilized at 2-8°C.
After reconstitution store the antibody undiluted at -20°C.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background The CD3 antigen is a protein complex composed of four distinct chains (CD3γ, CD3δ and two CD3ε) that associate with the T cell receptor (TCR) and the ζ-chain to generate an activation signal in T lymphocytes. The TCR, ζ-chain and CD3 molecules together comprise the TCR complex. The CD3γ, CD3δ and CD3ε chains are highly related cell surface proteins of the immunoglobulin superfamily containing a single extracellular immunoglobulin domain.
Synonyms T3/Leu-4
Reference Data

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