Interferon gamma (IFNG) Mouse Monoclonal Antibody [Clone ID: MD-1]

Specifications

Product Data
Clone Name	MD-1
Recommended Dilution	ELISA, ELISPOT, Flow cytometry, Immunohistochemistry, Neutralization assay, Western Blot
Reactivities	Macaque, Monkey, Human, Chimpanzee
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	IFN-gamma
Formulation	Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose.
Concentration	lot specific
Purification	Ion exchange chromatography.
Conjugation	Biotin
Storage	Store at -20°C as received.
Stability	Stable for 12 months from date of receipt.
Predicted Protein Size	16.7 kDa
Gene Name	interferon, gamma
Database Link	NP_000610 Entrez Gene 574282 MonkeyEntrez Gene 3458 Human UniProt ID P01579
Background	The neutralizing monoclonal antibody to monkey IFN-? (clone MD-1) was first described in 1985. The MD-1 antibody can be used in a sandwich ELISA and in ELISPOT assays (Aurisicchio L. et al. 2007, Koopman G. et al. 2008, Mäkitalo B. et al. 2002 and van der Meide P.H. et al. 1995). Further, the antibody has been descriped in immunohistochemical staining of frozen tissues of marmosets (Villoslada P. et al. 2000) and in intracellular flow cytometric analysis (Hamann D. et al. 1996). The MD-1 antibody has been reported to cross react with rhesus macaque, pig-tailed macaque, cynomogus monkey, baboon and African green monkey IFN-? and can be used in Luminex systems (Giavedoni L.D. 2005).
Synonyms	IFG; IFI
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Allograft rejection, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Proteasome, Regulation of autophagy, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Type I diabetes mellitus