CD3E Mouse Monoclonal Antibody [Clone ID: UCHT1]

Specifications

Product Data
Clone Name	UCHT1
Applications	FC
Recommended Dilution	Flow Cytometry: Use 10 µl of  neat antibody to label 106 cells or 100 µl whole blood
Reactivities	Human, Primate
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome. Spleen cells from immunised BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line.
Specificity	This antibody recognises a surface protein of peripheral blood T cells of molecular weight 19kD. As a pan T cell marker this antibody is present on more than 95% of circulating human peripheral T cells.
Formulation	PBS, pH 7.2 Label: APC State: Liquid purified IgG fraction Stabilizer: 1% BSA, 5% Sucrose Preservative: 0.09% Sodium Azide Label: Allophycocyanin
Reconstitution Method	Restore with distilled water
Purification	Affinity Chromatography on Protein G
Conjugation	APC
Storage	Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Database Link	Entrez Gene 916 Human UniProt ID P07766
Background	T Cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Synonyms	T3/Leu-4
Reference Data