CD62P (SELP) Mouse Monoclonal Antibody [Clone ID: AK-6]

Specifications

Product Data
Clone Name	AK-6
Applications	FC
Recommended Dilution	Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 106 cells in 100 µl. We recommend that this antibody be carefully titred against any previous batches to enable correct comparisons to be made with earlier results.
Reactivities	Human, Monkey
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Human platelet membrane glycoproteins.
Specificity	This antibody reacts with a 140 kD antigen expressed on activated platelets and also on endothelial cells both of blood and lymph vessels.
Formulation	PBS Label: FITC State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1 Absorption emission: 490 nm / 525 nm
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	selectin P
Database Link	Entrez Gene 6403 Human UniProt ID P16109
Background	CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
Synonyms	SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3
Reference Data