CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: LOB 3/17]

Specifications

Product Data
Clone Name	LOB 3/17
Applications	FC
Recommended Dilution	Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivities	Human, Monkey
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Fusion protein comprising extracellular domain of human Fas linked to human Fc. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Specificity	This antibody recognises the CD95 cell surface antigen.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein A.
Conjugation	FITC
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Database Link	Entrez Gene 355 Human UniProt ID P25445
Background	CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites. CD95 is expressed by activated T and B cells, NK cells and thymocytes.
Synonyms	FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6
Reference Data