HLA Class II DR Mouse Monoclonal Antibody [Clone ID: 423L]

Specifications

Product Data
Clone Name	423L
Applications	FC
Recommended Dilution	Flow Cytometry. Monitoring of B cells in peripheral blood. Analysis of B subsets. Study of B cell activation. Study of B cell neoplasms.
Reactivities	Human, Monkey
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human lymphoblastoid B-cell line RPMI 8866.
Specificity	This antibody recats with Human B and T subset Lymphocyte. Other species not tested.
Formulation	PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative. Label: FITC State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	FITC
Storage	Store undiluted at 2-8°C. DO NOT FREEZE!
Stability	Shelf life: one year from despatch.
Background	Identification of human B cell and T cell subsets associated with approximately 10% of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages.
Synonyms	HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR
Note	Protocol: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Reference Data