IL2 Rat Monoclonal Antibody [Clone ID: MQ1-17H12]

Specifications

Product Data
Clone Name	MQ1-17H12
Applications	FC
Recommended Dilution	Flow cytometry (neat - 1/10): Use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl. Membrane permeabilisation is required for this application.
Reactivities	Human, Monkey
Host	Rat
Isotype	IgG2a
Clonality	Monoclonal
Specificity	This antibody recognises interleukin-2 (IL-2) and may be used to detect intracellular IL-2 in flow cytometric techniques.
Formulation	PBS, pH 7.4, containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	FITC
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Protect from light. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	interleukin 2
Database Link	Entrez Gene 3558 Human UniProt ID P60568
Background	Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.
Synonyms	IL-2, TCGF
Reference Data