LOC104968698 Mouse Monoclonal Antibody [Clone ID: S100217]

Specifications

Product Data
Clone Name	S100217
Applications	FC, IF, IHC
Recommended Dilution	Immunohistochemistry: 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.  FFPE tissue section requires antigen retriever (boiling tissue in 10 mM citrate, pH 6.0 for 20 mins, followed by cooling at RT for 20 mins).  Immunocytochemistry.  Immunofluorescence: 10-20 µg/ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000). Recommended Positive Control: Melanoma or schwannoma.
Reactivities	Bovine, Human, Monkey, Mouse, Rat
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Purified bovine S100 protein
Specificity	This antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metasis. Cellular Localization: Cytoplasmic.
Formulation	PBS, pH 7.4 Label: FITC State: Liquid purified Ig fraction. Stabilizer: 1% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A Chromatography.
Conjugation	FITC
Storage	Store the antibody undiluted at 2-8°C. This product is photosensitive and should be protected from light.
Stability	Shelf life: One year from despatch.
Database Link	Entrez Gene 528735 Bovine UniProt ID P02639
Background	S100 protein is a type of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix ("EF-hand type") conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100% Soluble in ammonium sulfate at neutral pH. Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. To contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells.S-100 is normally present in cells derived from the neural crest (Schwann cells, melanocytes, and glia cells), chondrocyte, adipocytes, myoepithelial cells, macrophages, Langerhans cells, dendritic cells, and keratinocytes. It may be present in some breast epithelial cells.
Synonyms	S100 Calcium binding protein, S-100 protein, S100A, S100B, S-100 protein alpha chain, S-100 protein beta chain, Astrocyte Marker
Reference Data