IHC-plus™ TLR9 Antibody (aa268-284, FITC)
Référence LS-B2839-50
Conditionnement : 50ug
Marque : LSBio
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
Overview
Antibody:
TLR9 Mouse anti-Human Monoclonal (aa268-284) (FITC) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Horse
Format:
FITC, Unmodified
Specifications
Description
TLR9 antibody LS-B2839 is an FITC-conjugated mouse monoclonal antibody to TLR9 (aa268-284) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TLR9
Synonyms
TLR9 | CD289 | CD289 antigen | Scri2a | Toll-like receptor 9
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Dog, Horse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
FITC
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9).
Epitope
aa268-284
Specificity
Recognizes human TLR9. Detects a band of ~120kD by Western blot, may also detect a smaller band of TLR9 isoform B.
Applications
- IHC
- IHC - Paraffin (5 - 10 µg/ml)
- IHC - Frozen (10 - 20 µg/ml)
- ICC
- Western blot (1 - 3 µg/ml)
- Flow Cytometry
Usage
Immunohistochemistry: LS-B2839 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2839 was determined to be 5-10 ug/ml. Flow Cytometry (Intracellular): 0.1-2 ug/10^6 cells; cell surface. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
10 mM Tris, 150 mM NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee About TLR9
Q9NR96 NM_017442 NP_059138.1
Validation
Anti-TLR9 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
tech@hexabiogen.com