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> Mouse Monoclonal Anti-Vimentin, [Clone VIM 3B4] - FITC

Mouse Monoclonal Anti-Vimentin, [Clone VIM 3B4] - FITC

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Référence 61413

Conditionnement : 250µL

Marque : Progen

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Highly published
Diagnostic marker

anti-Vimentin mouse monoclonal, VIM 3B4, FITC Conjugate

anti-Vimentin mouse monoclonal, VIM 3B4, FITC Conjugate
Product: anti-Vimentin mouse monoclonal, VIM 3B4, FITC Conjugate
Art.No.: 61413

Quantity 250 µl
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate FITC
Application ELISA, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Amphibia, Bovine, Chicken, Dog, Human, Monkey
No reactivity Mouse
Storage 2-8°C
Intended use Research use only
Clone VIM 3B4
Immunogen Vimentin purified from bovine lens
Formulation Contains 0.09% sodium azide
UniprotID P48616 (Bovine), P09654 (Chicken), F1PLS4 (Dog, Canis familiaris), P08670 (Human)
Synonym Vimentin, VIM
Note Centrifuge prior to opening
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen At least 1:10
Immunohistochemistry (IHC) - paraffin At least 1:10 (protease treatment and/or microwave treatment recommended)
ELISA Assay dependent

The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin.
Polypeptide reacting: 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.
Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.
The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).

Tested cultured cell lines: fibroblasts (SV-80).

Bohn W, Wiegers W, Beuttenmüller M, Traub P: Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res 201: 1-7 (1992).
Rogers KR, Eckelt A, Nimmrich V, Janssen K-P, Schliwa M, Herrmann H, Franke WW: Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly. Eur J Cell Biol 66: 136-150 (1995).