TLR4 Mouse Monoclonal Antibody [Clone ID: HTA125]

Specifications

Product Data
Clone Name	HTA125
Applications	FC
Recommended Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 106 cells or 100 µl whole blood.
Reactivities	Canine, Guinea Pig, Human, Monkey, Porcine
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity	This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of SM1677LE for this purpose.
Formulation	PBS Label: FITC State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	Homo sapiens toll-like receptor 4 (TLR4), transcript variant 1
Database Link	Entrez Gene 574360 MonkeyEntrez Gene 7099 Human UniProt ID O00206
Background	TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
Synonyms	Toll-like receptor 4
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway