TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: 52B83]

CAT#: AM26298FC-N

TNF alpha (TNF) mouse monoclonal antibody, clone 52B83, FITC

Conjugation: Unconjugated FITC

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Specifications

Product Data
Clone Name 52B83
Applications IHC, WB
Recommended Dilution Immunohistochemistry on frozen sections (4): 6-µm tissue sections were fixed in acetone for 10 minutes (Ref. 4). 
Immunohistochemistry on paraffin sections (5): Tissue sections were fixed in 5% formalin embedded in paraffin and cut into 2µm sections (Ref. 5).
Flow cytometry (3): Antibody 52B83 was used to stain soluble TNF bound to TNF receptors.  Cells were fixed in PBA containing 0.2% formaldehyde (Ref. 3).
Western blot: A reduced sample treatment and 15% SDS-Page was used. The band size observed is 17 kDa (Ref.4).
The typical starting working dilution is 1:50.
Reactivities Guinea Pig, Human, Mouse, Monkey, Rhesus Monkey
Host Mouse
Isotype IgG1
Clonality Monoclonal
Specificity
The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha).
Formulation PBS
Label: FITC
State: Liquid 0.2 µm filtered Ig fraction
Stabilizer: 1% bovine serum albumin
Preservative: 0.02% sodium azide
Concentration lot specific
Purification Protein G
Conjugation FITC
Storage

Store at 2 - 8 °C.
Stability Shelf life: one year from despatch.
Gene Name tumor necrosis factor
Database Link
Background

TNF-alpha is a homotrimeric 17 kDa protein, that interacts with either one of the two types of TNF-receptors, termed I and II, leading to receptor cross-linking and signal transduction. The receptors differ strongly in their intra-cellular signaling pathways.
TNF-alpha was originally described as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-alpha is found to be a central mediator in many inflammatory and immunological processes. It can be induced by various products of micro-organisms and by various cytokines leading to expression of a wide variety of cytokines. The pro-inflammatory properties of TNF-alpha play a central role in several auto-immune diseases such as rheumatoid arthritis and inhibition by neutralizing molecules have been shown to be beneficial in patients.
Synonyms TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data

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  • Antibody and Western Standard
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