GRP94 antibody

Référence orb146771-200ug

Conditionnement : 200ug

Marque : Biorbyt

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GRP94 antibody

GRP94 antibody

Catalog Number: orb146771

Catalog Numberorb146771
CategoryAntibodies
DescriptionRat monoclonal to GRP94 (HRP). Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion. Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homology with its cytosolic equivalent, Hsp90. Both Hsp90 and Grp94 are calcium binding proteins. Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity. Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER..
Species/HostRat
ClonalityMonoclonal
Clone Number9G10
Tested applicationsFC, ICC, IP, WB
ReactivityBovine, Canine, Equine, Frog, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
IsotypeIgG2a
ImmunogenPurified Grp94 isolated from chicken oviducts
Concentration1 mg/ml
Dilution rangeWB (1:2000), ICC/IF (1:100)
ConjugationHRP
MW98kDa
TargetGRP94
Entrez7184
UniProt IDP14625
NCBINP_003290.1
StorageConjugated antibodies should be stored at 4°C
Buffer/PreservativesPBS pH 7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Alternative namesHSP90B1 antibody, GP96 antibody, TRA1 antibody, EC
NoteFor research use only
Application notes0.5 µg/ml of SMC-105 was sufficient for detection of Grp94 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Expiration Date12 months from date of receipt.