CD14 Mouse Monoclonal Antibody [Clone ID: UCHM1]

Specifications

Product Data
Clone Name	UCHM1
Applications	FC
Recommended Dilution	Flow cytometry.
Reactivities	Human, Monkey
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	Human Thymocytes followed by peripheral blood mononuclear cells. Spleen cells from an immunised mouse were fused with cells of a mouse myeloma line.
Specificity	This antibody recognises the CD14 cell surface antigen, a 55kD glycoprotein expressed by monocytes and macrophages.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide as preservative and 1% Bovine Serum Albumin as stabilizer. Label: PE State: Lyophilized purfied IgG fraction. Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with distilled water
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	PE
Storage	Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Gene Name	CD14 molecule
Database Link	Entrez Gene 929 Human UniProt ID P08571
Background	CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.
Synonyms	CD14
Reference Data