CD14 Mouse Monoclonal Antibody [Clone ID: MEM-15]

CAT#: SM3163R

CD14 mouse monoclonal antibody, clone MEM-15, PE


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Specifications

Product Data
Clone Name MEM-15
Applications FC
Recommended Dilution Flow cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension.
The content of a vial (2 ml) is sufficient for 100 tests.
Reactivities Human, Primate
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
Specificity This antibody reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.
The antibody also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
Formulation Phosphate buffered saline (PBS) solution containing 15mM sodium azide
Label: PE
State: Liquid purified Ig fraction
Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Conjugation PE
Storage Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Stability Shelf life: one year from despatch.
Gene Name CD14 molecule
Background CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.inflammatory and infectious processes.
Synonyms CD14
Reference Data

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