CD52 Rat Monoclonal Antibody [Clone ID: YTH34.5]

Specifications

Product Data
Clone Name	YTH34.5
Applications	FC
Recommended Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 1 x 10e6 cells.
Reactivities	Human, Monkey, Rhesus Monkey
Host	Rat
Isotype	IgG2b
Clonality	Monoclonal
Immunogen	Human lymphocytes.
Specificity	This antibody CD52 antibody, clone YTH34.5 reacts with the Human CD52 antigen, also known as CAMPATH-1.
Formulation	PBS Label: PE State: Lyophilized purified Ig fraction from Tissue Culture Supernatant Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1 ml distilled water.
Purification	Affinity Chromatography on Protein A
Conjugation	PE
Storage	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Database Link	Entrez Gene 1043 Human UniProt ID P31358
Background	CD52 is a small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link (Xia et al. 1991). The apparent molecular mass of the native antigen on SDS-PAGE is 25-29kDa, considerably reduced following N-glycanase treatment (Rowan et al. 1998). CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies (Dearden et al. 2002; Pettitt et al. 2012).
Synonyms	HE5, CDw52
Reference Data