Specifications

Product Data
Clone Name	FMC46
Applications	FC
Recommended Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 106 cells or 100 µl whole blood.
Reactivities	Bovine, Human, Monkey
Host	Mouse
Isotype	IgG2b
Clonality	Monoclonal
Immunogen	PHA stimulated lymphoblasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognizes CD62L, also known a L-selectin.
Formulation	PBS Label: PE State: Lyophilized purified IgG Stabilizer: 1% BSA, 5% Sucrose Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin (RPE) Absorption emission: 496 nm / 578 nm (RPE 488nm laser)
Reconstitution Method	Reconstitute with 0.25ml distilled water
Purification	Affinity chromatography on Protein G
Conjugation	PE
Storage	Store undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Gene Name	selectin L
Database Link	Entrez Gene 6402 Human UniProt ID P14151
Background	CD62L is a 74-95kDa member of the selectin family of adhesion receptors, which acts as a ligand for both CD62-P (P-selectin) and CD62E (E-selectin). Human CD62L is constitutively expressed on most leucocytes including monocytes, granulocytes, lymphocytes, NK cells, bone marrow myeloid progenitor cells and on a subset of thymocytes. CD62L plays an important role in leucocyte tethering and rolling on the endothelial cell surface and for the homing of naive lymphocytes to lymph nodes and Peyers patches via HEV. Neutrophils require a constant supply of this molecule on the cell surface for migration into peripheral tissues and adhesion to activated endothelium at sites of inflammation, where CD62L is rapidly shed as soluble L-selectin, but surface expression still remains. The expression of CD62L is down regulated on lymphocytes and neutrophils by PMA stimulation.
Synonyms	SELL, LNHR, LYAM1, Leu-8, TQ1, gp90-MEL, LECAM1, LAM-1
Reference Data