Integrin alpha 6 (ITGA6) Rat Monoclonal Antibody [Clone ID: NKI-GoH3]

Specifications

Product Data
Clone Name	NKI-GoH3
Applications	FC
Recommended Dilution	Flow Cytometry: Neat - 1/5; Use 10µl of the suggested working dilution to label 10e6 platelets in 100µl.
Reactivities	Canine, Human, Monkey, Mouse, Porcine
Host	Rat
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.
Specificity	This antibody recognises CD49F (VLA-6 alpha chain). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin. We recommend the use of SM038Afor this purpose.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: PE State: Lyophilized purified IgG Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1.0 ml distilled water.
Purification	Affinity chromatography on Protein G
Conjugation	PE
Storage	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE!
Stability	Shelf life: one year from despatch.
Gene Name	integrin subunit alpha 6
Database Link	Entrez Gene 3655 Human UniProt ID P23229
Background	CD49F (VLA-6 alpha chain)is a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1).
Synonyms	Integrin alpha-6, VLA-6, VLA6
Reference Data