CD34 Mouse Monoclonal Antibody [Clone ID: 581]

Specifications

Product Data
Clone Name	581
Applications	FC
Recommended Dilution	Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivities	Human, Primate (Does not react with: Sheep)
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Specificity	This antibody reacts with CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease.
Formulation	Phosphate buffered saline (PBS) Label: PerCP State: Liquid purified Ig fraction Preservative: 15 mM sodium azide Label: Conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Conjugation	PerCP
Storage	Store undiluted at 2-8°C. DO NOT FREEZE! This products is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Database Link	Entrez Gene 947 Human UniProt ID P28906
Background	CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Synonyms	Hematopoietic progenitor cell marker
Reference Data