Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

Anti-MEK-1/2 antibody (160-240)

Imprimer

Référence NB-22-4078-200ul

Conditionnement : 200µg

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Marque : Neo Biotech

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of various cells using MEK-1/2 Polyclonal Antibody diluted at 1：1000
Immunofluorescence analysis of rat-lung tissue. 1, MEK-1/2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of mouse-kidney tissue. 1, MEK-1/2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of mouse-kidney tissue. 1, MEK-1/2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of mouse-lung tissue. 1, MEK-1/2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of mouse-lung tissue. 1, MEK-1/2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. 1, MEK-1/2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, MEK-1/2 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of lysates from COS7/HT-29, using MEK1/2 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of Hela cell. 1, MEK-1/2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CYCS monoclonal antibody (4B10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

General Info

Host:	Rabbit
Applications:	IF/WB/IHC/ELISA
Reactivity:	Human/Mouse/Rat/Monkey
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Dual specificity mitogen-activated protein kinase kinase 1 and Dual specificity mitogen-activated protein kinase kinase 2 (189-238 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and E

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	IF 1:50-200 WB 1:500-2000 ELISA 1:10000-20000 IHC 1:50-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	MAP2K1 MAP2K2
Gene ID:	5604 5605
Uniprot ID:	MP2K1_HUMAN MP2K2_HUMAN
Immunogen Region:	189-238 aa
Specificity:	MEK-1/2 Polyclonal Antibody detects endogenous levels of MEK-1/2 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human MEK1/2 at the amino acid range 189-238

Description

Post Translational Modifications	Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Autophosphorylated at Ser-218 and Ser-222, autophosphosphorylation is promoted by NEK10 following UV irradiation. (Microbial infection) Acetylation by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Function	Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner.by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Protein Name	Dual Specificity Mitogen-Activated Protein Kinase Kinase 1 Map Kinase Kinase 1 Mapkk 1 Mkk1 Erk Activator Kinase 1 Mapk/Erk Kinase 1 Mek 1
Database Links	Reactome: R-HSA-110056 Reactome: R-HSA-170968 Reactome: R-HSA-445144 Reactome: R-HSA-5210891 Reactome: R-HSA-5673000 Reactome: R-HSA-5674135 Reactome: R-HSA-5674499 Reactome: R-HSA-5684264 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-9649948 Reactome: R-HSA-9652169 Reactome: R-HSA-9656223
Cellular Localisation	Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Spindle Pole Body Nucleus Membrane Peripheral Membrane Protein Localizes At Centrosomes During Prometaphase Midzone During Anaphase And Midbody During Telophase/Cytokinesis Membrane Localization Is Probably Regulated By Its Interaction With Ksr1
Alternative Antibody Names	Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 1 antibody Anti-Map Kinase Kinase 1 antibody Anti-Mapkk 1 antibody Anti-Mkk1 antibody Anti-Erk Activator Kinase 1 antibody Anti-Mapk/Erk Kinase 1 antibody Anti-Mek 1 antibody Anti-MAP2K1 antibody Anti-MEK1 antibody Anti-PRKMK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance