General Info
| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Phospho-B-cell linker protein-Tyr96 (62-111 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | BLNK |
| Gene ID: | 29760 |
| Uniprot ID: | BLNK_HUMAN |
| Immunogen Region: | 62-111 aa |
| Specificity: | Phospho-BLNK (Y96) Polyclonal Antibody detects endogenous levels of BLNK protein only when phosphorylated at Y96. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human BLNK around the phosphorylation site of Tyr96 at the amino acid range 62-111 |
Description
| Post Translational Modifications | Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways. |
| Function | Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis. |
| Protein Name | B-Cell Linker ProteinB-Cell Adapter Containing A Sh2 Domain ProteinB-Cell Adapter Containing A Src Homology 2 Domain ProteinCytoplasmic Adapter ProteinSrc Homology 2 Domain-Containing Leukocyte Protein Of 65 KdaSlp-65 |
| Database Links | Reactome: R-HSA-912631Reactome: R-HSA-9679191Reactome: R-HSA-983695 |
| Cellular Localisation | CytoplasmCell MembraneBcr Activation Results In The Translocation To Membrane Fraction |
| Alternative Antibody Names | Anti-B-Cell Linker Protein antibodyAnti-B-Cell Adapter Containing A Sh2 Domain Protein antibodyAnti-B-Cell Adapter Containing A Src Homology 2 Domain Protein antibodyAnti-Cytoplasmic Adapter Protein antibodyAnti-Src Homology 2 Domain-Containing Leukocyte Protein Of 65 Kda antibodyAnti-Slp-65 antibodyAnti-BLNK antibodyAnti-BASH antibodyAnti-SLP65 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance