Anti-Phospho-MITF-Ser180 antibody (120-200)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	MITF
Gene ID:	4286
Uniprot ID:	MITF_HUMAN
Immunogen Region:	151-200 aa
Specificity:	Phospho-MITF (S180) Polyclonal Antibody detects endogenous levels of MITF protein only when phosphorylated at S180.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human MITF around the phosphorylation site of Ser180/73 at the amino acid range 151-200

Post Translational Modifications	When nutrients are present, phosphorylation by MTOR at Ser-5 via non-canonical mTORC1 pathway promotes ubiquitination by the SCF(BTRC) complex, followed by degradation. Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. Phosphorylation by MARK3/cTAK1 at Ser-280 promotes association with 14-3-3/YWHA adapters and retention in the cytosol. Phosphorylated at Ser-180 and Ser-516 following KIT signaling, triggering a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome. Phosphorylated in response to blue light (415nm). Ubiquitinated by the SCF(BTRC) and SCF(FBXW11) complexes following phosphorylation ar Ser-5 by MTOR, leading to its degradation by the proteasome. Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome. Deubiquitinated by USP13, preventing its degradation.
Function	Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR). Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity. Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Protein Name	Microphthalmia-Associated Transcription Factor Class E Basic Helix-Loop-Helix Protein 32 Bhlhe32
Database Links	Reactome: R-HSA-3232118
Cellular Localisation	Nucleus Cytoplasm Lysosome Membrane When Nutrients Are Present Recruited To The Lysosomal Membrane Via Association With Gdp-Bound Ragc/Rragc (Or Ragd/Rragd): It Is Then Phosphorylated By Mtor Phosphorylation By Mtor Promotes Ubiquitination And Degradation Conversely Inhibition Of Mtorc1 Starvation And Lysosomal Disruption Promotes Dephosphorylation And Translocation To The Nucleus Phosphorylation By Mark3/Ctak1 Promotes Association With 14-3-3/Ywha Adapters And Retention In The Cytosol
Alternative Antibody Names	Anti-Microphthalmia-Associated Transcription Factor antibody Anti-Class E Basic Helix-Loop-Helix Protein 32 antibody Anti-Bhlhe32 antibody Anti-MITF antibody Anti-BHLHE32 antibody

Information sourced from Uniprot.org

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