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> Anti-Phospho-SF1-Ser82 antibody (20-100)

Anti-Phospho-SF1-Ser82 antibody (20-100)

Imprimer Imprimer
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Référence NB-22-1054-200ul

Conditionnement : 200µg

Marque : Neo Biotech

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Téléphone : +1 850 650 7790

  • Western blot analysis of lysates from HUVEC cells treated with anisomycin 25ug/ml 30', using SF1 (Phospho-Ser82) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of A549 cells, using SF1 (Phospho-Ser82) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using SF1 (Phospho-Ser82) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of SH-SY5Y 293T using p-Splicing factor 1 (S82) antibody. Antibody was diluted at 1:500

General Info

Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Splicing factor 1-Ser82 (48-97 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: SF1
Gene ID: 7536
Uniprot ID: SF01_HUMAN
Immunogen Region: 48-97 aa
Specificity: Phospho-Splicing factor 1 (S82) Polyclonal Antibody detects endogenous levels of Splicing factor 1 protein only when phosphorylated at S82.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SF1 around the phosphorylation site of Ser82 at the amino acid range 48-97

Description

Post Translational Modifications Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463.
Function Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
Protein Name Splicing Factor 1
Mammalian Branch Point-Binding Protein
Bbp
Mbbp
Transcription Factor Zfm1
Zinc Finger Gene In Men1 Locus
Zinc Finger Protein 162
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Splicing Factor 1 antibody
Anti-Mammalian Branch Point-Binding Protein antibody
Anti-Bbp antibody
Anti-Mbbp antibody
Anti-Transcription Factor Zfm1 antibody
Anti-Zinc Finger Gene In Men1 Locus antibody
Anti-Zinc Finger Protein 162 antibody
Anti-SF1 antibody
Anti-ZFM1 antibody
Anti-ZNF162 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance