Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	IF 1:50-200 WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:40000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	YAP1
Gene ID:	10413
Uniprot ID:	YAP1_HUMAN
Immunogen Region:	93-142 aa
Specificity:	Phospho-YAP (S127) Polyclonal Antibody detects endogenous levels of YAP protein only when phosphorylated at S127.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human YAP around the phosphorylation site of Ser127 at the amino acid range 93-142

Post Translational Modifications	Phosphorylated by LATS1 and LATS2.leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1.leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes.in response to DNA damage. Phosphorylation at Ser-400 and Ser-403 by CK1 is triggered by previous phosphorylation at Ser-397 by LATS proteins and leads to YAP1 ubiquitination by SCF(beta-TRCP) E3 ubiquitin ligase and subsequent degradation. Phosphorylated at Thr-119, Ser-138, Thr-154, Ser-367 and Thr-412 by MAPK8/JNK1 and MAPK9/JNK2, which is required for the regulation of apoptosis by YAP1. Ubiquitinated by SCF(beta-TRCP) E3 ubiquitin ligase.
Function	Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization. Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1. In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. Isoform 2: Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3). Isoform 3: Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).
Protein Name	Transcriptional Coactivator Yap1 Yes-Associated Protein 1 Protein Yorkie Homolog Yes-Associated Protein Yap65 Homolog
Database Links	Reactome: R-HSA-1251985 Reactome: R-HSA-2028269 Reactome: R-HSA-2032785 Reactome: R-HSA-8939236 Reactome: R-HSA-8940973 Reactome: R-HSA-8951671 Reactome: R-HSA-9619665 Reactome: R-HSA-9796292
Cellular Localisation	Cytoplasm Nucleus Cell Junction Both Phosphorylation And Cell Density Can Regulate Its Subcellular Localization Phosphorylation Sequesters It In The Cytoplasm By Inhibiting Its Translocation Into The Nucleus At Low Density Predominantly Nuclear And Is Translocated To The Cytoplasm At High Density Ptpn14 Induces Translocation From The Nucleus To The Cytoplasm Localized Mainly To The Nucleus In The Early Stages Of Embryo Development With Expression Becoming Evident In The Cytoplasm At The Blastocyst And Epiblast Stages
Alternative Antibody Names	Anti-Transcriptional Coactivator Yap1 antibody Anti-Yes-Associated Protein 1 antibody Anti-Protein Yorkie Homolog antibody Anti-Yes-Associated Protein Yap65 Homolog antibody Anti-YAP1 antibody Anti-YAP65 antibody

Information sourced from Uniprot.org

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