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Mouse Monoclonal Anti-Carcinoembryonic Antigen (CEA) / CD66 [Clone CEA31]
Référence NB-36-00030-P1
Conditionnement : 100ug
Marque : Neo Biotech
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Printable Datasheet
Carcinoembryonic Antigen (CEA) / CD66 Antibody [CEA31]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human colon carcinoma stained with CEA Mouse Monoclonal Antibody (CEA31).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with CEA Mouse Monoclonal Antibody (CEA31).
SDS-PAGE Analysis of Purified CEA Mouse Monoclonal Antibody (CEA31). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with CEA Mouse Monoclonal Antibody (CEA31).
Applications & Dilutions
Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Monkey
Research Areas
Hematopoietic Stem Cells
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Cell surface
Gene Name
CEACAM5
Positive Control
MCF7 or 293T cells. Human colon carcinoma.
Immunogen
Human colon carcinoma extract
Alternate Names
Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen, Meconium antigen 100, Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)
Functions
- Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:2803308, PubMed:10910050, PubMed:10864933). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050).
- (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
Key References
- Muraro R, et. al. Cancer Research, 1985, 45:5769-80.
- Siler K, et. al. Biotechnology Therapeutics, 1993, 4(3-4):163-81.
- Robbins PF, et. al. International Journal of Cancer, 1993, 53(6):892-7.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/3670312/
- https://pubmed.ncbi.nlm.nih.gov/3220478/
- https://pubmed.ncbi.nlm.nih.gov/2342461/
- https://pubmed.ncbi.nlm.nih.gov/15057824/
- https://pubmed.ncbi.nlm.nih.gov/3814146/
- https://pubmed.ncbi.nlm.nih.gov/3033671/
- https://pubmed.ncbi.nlm.nih.gov/2803308/
- https://pubmed.ncbi.nlm.nih.gov/2317824/
- https://pubmed.ncbi.nlm.nih.gov/10436421/
- https://pubmed.ncbi.nlm.nih.gov/10910050/
- https://pubmed.ncbi.nlm.nih.gov/10864933/
- https://pubmed.ncbi.nlm.nih.gov/16740002/
- https://pubmed.ncbi.nlm.nih.gov/19159218/
- https://pubmed.ncbi.nlm.nih.gov/26483485/
- https://pubmed.ncbi.nlm.nih.gov/10854848/
- https://pubmed.ncbi.nlm.nih.gov/18086185/
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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