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Mouse Monoclonal Anti-CD44 / HCAM Std. [Clone 156-3C11]
Référence NB-36-00996-P0
Conditionnement : 20ug
Marque : Neo Biotech
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Printable Datasheet
CD44 / HCAM Std. (Cancer Stem Cell Marker) Antibody [156-3C11]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Tonsil stained with CD44 Mouse Monoclonal Antibody (156-3C11).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CD44 Mouse Monoclonal Antibody (156-3C11).
SDS-PAGE Analysis of Purified CD44 Mouse Monoclonal Antibody (156-3C11). Confirmation of Purity and Integrity of Antibody.
Confocal immunofluorescence image of HeLa cells using CD44 Mouse Monoclonal Antibody (156-3C11) Green (CF488) and Reddot is used to label the nuclei Red.
Western Blot Analysis of HeLa cell lysate using CD44 Mouse Monoclonal Antibody (156-3C11).
CD44 Antibody in Western Blot (WB)
Western Blot Analysis of MDA-MB-231 lysate using CD44 Mouse Monoclonal Antibody (156-3C11)
Applications & Dilutions
Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol
Summary
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Baboon, Green Monkey, Human
Research Areas
Cardiovascular, Immunology, Articular Cartilage Extracellular Matrix, Cytokine Signaling, Hematopoietic Stem Cells, Mesenchymal Stem Cell Differentiation, Neural Stem Cells
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell membrane, Cell projection, Microvillus
Gene Name
CD44
Positive Control
HeLa cells, MDA-MB-231, Paracortex in tonsil or human Breast Carcinoma
Immunogen
Stimulated human leukocytes
Alternate Names
CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, LHR; BA-1; chondroitin sulfate proteoglycan 8 (CSPG8); Epican; Extracellular Matrix Receptor III (ECM III); GP90 Lymphocyte Homing Adhesion Receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan Sulfate Proteoglycan; Hermes Antigen; HAS; HUTCH I; Hyaluronate Receptor; Indian blood group; Inlu Related p80 Glycoprotein; Ly 24; MDU2; MDU3; MIC4; MUTCH I; Phagocytic Glycoprotein 1 (PGP-1)
Functions
- Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).
Key References
- Schlossman SF, et. al. Leucocyte Typing V, p1713-1719, Oxford Univ. Press, 1995.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/2466575/
- https://pubmed.ncbi.nlm.nih.gov/1840487/
- https://pubmed.ncbi.nlm.nih.gov/1991450/
- https://pubmed.ncbi.nlm.nih.gov/2056274/
- https://pubmed.ncbi.nlm.nih.gov/1281868/
- https://pubmed.ncbi.nlm.nih.gov/1465456/
- https://pubmed.ncbi.nlm.nih.gov/7508842/
- https://pubmed.ncbi.nlm.nih.gov/8352881/
- https://pubmed.ncbi.nlm.nih.gov/10933060/
- https://pubmed.ncbi.nlm.nih.gov/17974005/
- https://pubmed.ncbi.nlm.nih.gov/16554811/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/1922057/
- https://pubmed.ncbi.nlm.nih.gov/2466576/
- https://pubmed.ncbi.nlm.nih.gov/7527301/
- https://pubmed.ncbi.nlm.nih.gov/7508992/
- https://pubmed.ncbi.nlm.nih.gov/2007624/
- https://pubmed.ncbi.nlm.nih.gov/8148709/
- https://pubmed.ncbi.nlm.nih.gov/1717145/
- https://pubmed.ncbi.nlm.nih.gov/7528188/
- https://pubmed.ncbi.nlm.nih.gov/12511867/
- https://pubmed.ncbi.nlm.nih.gov/9580567/
- https://pubmed.ncbi.nlm.nih.gov/12032545/
- https://pubmed.ncbi.nlm.nih.gov/12883358/
- https://pubmed.ncbi.nlm.nih.gov/15123640/
- https://pubmed.ncbi.nlm.nih.gov/16335952/
- https://pubmed.ncbi.nlm.nih.gov/17081983/
- https://pubmed.ncbi.nlm.nih.gov/16541107/
- https://pubmed.ncbi.nlm.nih.gov/19367720/
- https://pubmed.ncbi.nlm.nih.gov/18691976/
- https://pubmed.ncbi.nlm.nih.gov/18669648/
- https://pubmed.ncbi.nlm.nih.gov/19159218/
- https://pubmed.ncbi.nlm.nih.gov/19381274/
- https://pubmed.ncbi.nlm.nih.gov/19690332/
- https://pubmed.ncbi.nlm.nih.gov/19703720/
- https://pubmed.ncbi.nlm.nih.gov/18757307/
- https://pubmed.ncbi.nlm.nih.gov/20962267/
- https://pubmed.ncbi.nlm.nih.gov/20068231/
- https://pubmed.ncbi.nlm.nih.gov/21269460/
- https://pubmed.ncbi.nlm.nih.gov/21406692/
- https://pubmed.ncbi.nlm.nih.gov/22171320/
- https://pubmed.ncbi.nlm.nih.gov/22726066/
- https://pubmed.ncbi.nlm.nih.gov/23589287/
- https://pubmed.ncbi.nlm.nih.gov/23186163/
- https://pubmed.ncbi.nlm.nih.gov/24275569/
- https://pubmed.ncbi.nlm.nih.gov/25944712/
- https://pubmed.ncbi.nlm.nih.gov/29777959/
- https://pubmed.ncbi.nlm.nih.gov/14992719/
- https://pubmed.ncbi.nlm.nih.gov/17085435/
- https://pubmed.ncbi.nlm.nih.gov/25195884/
- https://pubmed.ncbi.nlm.nih.gov/8636151/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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