β Tubulin (2-28-33) AC

Référence sc-23949 AC

Conditionnement : 500µg/ml,25%ag

Marque : Santa Cruz Biotechnology

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Anticorps beta Tubulin (2-28-33): sc-23949

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Fiches techniques
  • L'Anticorps beta Tubulin (2-28-33) est un monoclonal de souris IgG1 κ, cité dans 37 publications, fourni en 200 µg/ml
  • raised against Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (Sea Urchin) sperm axonemes
  • Anti-L'Anticorps beta Tubulin (2-28-33) est recommandé pour la détection de β Tubulin d'origine mammalian species, zebrafish, Drosophila et Xenopus par WB, IP, IF et IHC(P)
  • Anti-L'Anticorps beta Tubulin (2-28-33) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
  • m-IgG Fc BP-HRP et 1 BP-HRP">m-IgG1 BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps beta Tubulin (2-28-33) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps beta Tubulin (2-28-33) ().

ACCÈS RAPIDE AUX LIENS

, α 3 Tubulin, α 4 Tubulin, α Tubulin, β Tubulin, β 2 Tubulin, β 3 Tubulin, δ Tubulin, ε Tubulin et γ Tubulin

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β Tubulin Antibody (2-28-33) is an IgG1 κ mouse monoclonal β Tubulin antibody that detects β Tubulin of mammalian species, zebrafish, Drosophila and Xenopus origin by WB, IP, IF and IHC(P). β Tubulin Antibody (2-28-33) is available as the non-conjugated anti-β Tubulin antibody form, as well as multiple conjugated forms of anti-β Tubulin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

I am using β Tubulin (2-28-33): sc-23949 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Posée par: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 3 de 5 de mmassimi par Good features. I tested the antibody on Western blot with Huh-7 cells; it worked fine, producing a fairly clean signal but only at a relatively low dilution, i.e. 1: 200.
Date de publication: 2021-05-05
Rated 5 de 5 de kking03 par works great in zebrafish Used in zebrafish ICC and it worked beautifully, used at 1:100, haven't used in whole mount yet but assuming it will work the same
Date de publication: 2018-02-26
Rated 4 de 5 de Redder par Worked for WB It gives good bands at 1:500 dilution for BEAS2B cells.
Date de publication: 2018-01-04
Rated 5 de 5 de Meng fei par Sucessful loading control This antibody always been used for loading control,I loading 20ug mouse brain tissue ,got strong band at 1:2000.
Date de publication: 2017-02-08
Rated 5 de 5 de Randy McDonald par Nice western blot results We used this antibody for a western blot experiment and got nice dark bands at the correct molecular weight
Date de publication: 2017-01-19
Rated 4 de 5 de Geoff par Works well in Western Blot applications using Works well in Western Blot applications using A547 and UCI-107 cell lines.
Date de publication: 2015-05-11
Rated 5 de 5 de SH par Specific and sensitive for WB Specific and sensitive for WB, works well in K-562. -SCBT QC
Date de publication: 2015-01-26
Rated 5 de 5 de JM par Works well for immunofluorescence with HeLa Works well for immunofluorescence with HeLa samples. -SCBT QC
Date de publication: 2014-03-07
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Anticorps beta Tubulin (2-28-33) est évalué 4.5 de 5 de 8.
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