acetylated α Tubulin (6-11B-1) AC

Référence sc-23950 AC

Conditionnement : 500µg/ml,25%ag

Marque : Santa Cruz Biotechnology

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Anticorps acetylated alpha Tubulin (6-11B-1): sc-23950

Fiches techniques
  • L'anticorps acetylated alpha Tubulin 6-11B-1 est un monoclonal IgG2b κ L'Anticorps acetylated alpha Tubulin, cité dans 311 publications, fourni en 200 µg/ml
  • raised against the outer arms of Strongylocentrotus purpuratus (Sea Urchin) sperm axonemes
  • L'Anticorps acetylated alpha Tubulin (6-11B-1) est recommandé pour la détection de acetylated α Tubulin d'origine mammalian species, zebrafish, Drosophila et Xenopus par WB, IP, IF et IHC(P); non cross-reactif avec non-acetylated α Tubulin or other lysine acetylation sites
  • Anti-L'Anticorps acetylated alpha Tubulin (6-11B-1) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
  • m-IgG Fc BP-HRP et 2b BP-HRP">m-IgG2b BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps acetylated alpha Tubulin (6-11B-1) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps acetylated alpha Tubulin (6-11B-1) ().

ACCÈS RAPIDE AUX LIENS

, α 3 Tubulin, α 4 Tubulin, α Tubulin, β Tubulin, β 2 Tubulin, β 3 Tubulin, δ Tubulin, ε Tubulin et γ Tubulin

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acetylated α Tubulin Antibody (6-11B-1) is an IgG2b κ mouse monoclonal acetylated alpha Tubulin antibody that detects the acetylated alpha Tubulin protein of mammalian species, zebrafish, Drosophila and Xenopus origin by WB, IP, IF and IHC(P). acetylated α Tubulin Antibody (6-11B-1) is available as both the non-conjugated anti-acetylated alpha Tubulin antibody form, as well as multiple conjugated forms of anti-acetylated alpha Tubulin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

We have lot # HQ422 of prodfuct sc-23950. I am getting a very week signal at 1:200 on fixed airway epithelial cells. Is this lot expired? Thanks. Shubha

Posée par: Shubha
Thank you for your question. We warranty our antibodies for 1 year from date of purchase . Lot HQ422 is not a valid lot number . Please give us a call or email us to discuss your results. [email protected] or (800)457-3801.
Répondue par: BlakeJ
Date de publication: 2023-12-01

The product specifications list amino acids 149-448, however, in previous questions it was noted that this product recognizes Ac-K40. Can you please confirm which epitope this antibody recognizes?

Posée par: Laura
Thank you for your question. The epitope range is 149-448.
Répondue par: BlakeJ
Date de publication: 2023-11-14

Hi, interested in buying an anti-acetyl-alpha Tubulin antibody to test the effect of HDAC8 inhibitors by WB. Wondering if I could purchase or get an small aliquot to test it before placing the order of a full product

Posée par: CarlosPR
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Répondue par: Dallas Tech Service
Date de publication: 2021-04-07

We have purchased and used acetylated α Tubulin Antibody (6-11B-1): sc-23950 antibody. It is working quite well. However we need to know the which amino acid residue the antibody is reacting with? Kindly tell me particular epitope to which ab binds.

Posée par: Nitish
Thank you for your question. This mouse monoclonal antibody recognizes endogenous levels of α-tubulin protein only when acetylated at Lys40 (Lysine 40).
Répondue par: Technical Support
Date de publication: 2019-05-03

Hi, I would like to know which light chain is in this antibody (e.g, kappa I, II or V). I would like to undertsand if I can purify the antibody with protein L resin. I would like to find out the affinity of the antibody with protein L. Thank you!

Posée par: Foram
Thank you for your question. Since acetylated α Tubulin Antibody (6-11B-1): sc-23950 is a mouse IgG2 antibody it can be purified using Protein A or Protein A/G. For further information please contact our technical service directly.
Répondue par: Technical Support Europe
Date de publication: 2019-02-04

Hi, I am interested in co-staining all microtubules (a-tubulin ab) and acetylated ones (acetylated a-tubulin). The issue is that both are mouse. Would a mouse secondary detect a conjugated acetylated a-tub antibody?

Posée par: JuanManuelGomez
Thank you for your question. We would not recommend co-staining with one conjugated primary antibody, and one un-conjugated primary antibody combined with an anti-mouse secondary antibody. There would still be the possibility of cross-reactivity between the anti-mouse IgG and the conjugated mouse monoclonal antibody. Instead, we would recommend co-staining with two conjugated primary antibodies, if both are mouse IgG.
Répondue par: Technical Support
Date de publication: 2017-05-16

Dear Santa Cruz! I would like to stain the primary cilium of HK2 cells with sc-23950. I saw a cytoskeletal network on one picture and individual cilia on the other. How can I achieve the cilia staining, with no visibility of the cytoskeleton? Thank you

Posée par: mkeszthelyi
Thank you for your question. We do not have a specific protocol for using our monoclonal acetylated α Tubulin Antibody (6-11B-1): sc-23950 for detection of individual cilium exclusively. Provided below is a link to our recommended protocol for immunofluorescence and immunoperoxidase staining. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining This antibody has also been cited in the literature at least 30 times, many of which are using the antibody for IF or IHC applications. For example, PMID's: 27062996, 26079437 and 25286302. We hope that some of these publications may be useful in identifying a protocol for your research needs.
Répondue par: Technical Support
Date de publication: 2017-03-01

What is the acetylation site that is recognized by this antibody?  Will it detect non-acetylated α Tubulin?

Posée par: Dr Ninau Qelp
acetylated α Tubulin Antibody (6-11B-1): sc-23950 recognizes endogenous levels of α-tubulin protein only when acetylated at Lys40.
Répondue par: Tech Service 2
Date de publication: 2016-12-15
  • Anticorps acetylated alpha Tubulin (6-11B-1) Questions - page 2
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Rated 5 de 5 de akire par muy buen reconocimiento de la proteína Lo probé con una linea celular de mosquito y reconoció la proteína muy bien
Date de publication: 2023-10-03
Rated 5 de 5 de bkasp par Acet. alpha-tubulin Acet. Alpha-tubulin AlexaFluor 488 antibody was used 1/100 diluted in D-PBS/Live-Dead stain (1:1000), supplemented with 60µg/ml human IgG (Sigma Aldrich) at 8◦C for 30 min. After that, cells were washed in FACS buffer (D-PBS/0.1% BSA), fixed in 4% PFA at room temperature (RT), and analyzed by flow cytometry.
Date de publication: 2023-01-16
Rated 5 de 5 de nevermind par GREAT ANTIBODIES! Really good antibody. Whole cell lysate, Primary: 1:200, Secondary: 1:4000
Date de publication: 2022-06-14
Rated 5 de 5 de dfnuo par Good antibody for ac-tubulin Good antibody for ac-tubulin detection in WB.Very clean and strong signal.
Date de publication: 2020-11-17
Rated 5 de 5 de Ratatouille5 par Primary cilia - Immunofluorescence The antibody works quite well at 1/1000 in fixed cells and is highly specific.
Date de publication: 2019-07-29
Rated 5 de 5 de R1512 par Primary cilia - Immunofluorescence The antibody works quite well and is specific to the target.
Date de publication: 2019-05-28
Rated 5 de 5 de arndmrutgers par Very good for WB and IF I got a sample for test. It has very nice signal in western Blot. I loved the immunofluorescence images. Now I bought 1ml of this antibody and it works great.
Date de publication: 2019-04-22
Rated 4 de 5 de pollar par good signal in IF experiments this antibody can give a good IF signal in some tissues.
Date de publication: 2019-02-05
  • Anticorps acetylated alpha Tubulin (6-11B-1) Avis - page 2
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Anticorps acetylated alpha Tubulin (6-11B-1) est évalué 4.8 de 5 de 43.
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