ADH1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommended Dilution	Western blot: 1/1,000-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/4,000-1/20,000. Immunohistochemistry on Paraffin (FFPE) or Frozen sections: 1/500-1/2,500.
Reactivities	Yeast
Host	Rabbit
Clonality	Polyclonal
Immunogen	Alcohol dehydrogenase from yeast
Specificity	This antibody detects yeast alcohol dehydrogenase. Cross reactivity against alcohol dehydrogenase from other sources may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified yeast alcohol dehydrogenase.
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig faction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Database Link	UniProt ID P00330
Background	Alcohol Dehydrogenases (ADH) are a group of dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of nicotinamide adenine dinucleotide (NAD+ to NADH). In humans and many other animals, they serve to break down alcohols that otherwise are toxic, and they also participate in generation of useful aldehyde, ketone, or alcohol groups during biosynthesis of various metabolites.
Synonyms	Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, YOL086C, O0947
Reference Data