18 month shelf life at 2°C to 8°C.Mixed working reagent is stable for several days at room temperature or at 4°C. Exceptional lot to lot consistency. Superior stability.
Tested applications:
ELISA (ELISA), Microwell application (MA)
Additional Information
Additional information:
This reagent offers extreme low femto level sensitivity combined with low background and superior signal to noise ratios
Additional information (application):
User Instruction
Store reagents A and B in the darkness at 4-8°C.
Mix equal volumes of reagent A and B (chemiluminescent substrate) in a clean container and equilibrate to room temperature 30 minutes before use.
Do not contaminate the HRP enzyme substrate with HRP or other proteins. Never pipette directly from the bottle stock or pour aliquoted solution back into the stock bottle.
100 µl of Agrisera ELISA-ECL should be used/well.
Incubate at RT for optimial detection e.g. for example 20 min. after substrate contact.
Assay in appropriate microtieter plates designed for chemiluminescence.
Detection conditions need to be optimized for each assay system. This reagent is extremely sensitive and very high HRP concentration can "burn out" the substtrate. This substrate has a very wide detection range for HRP enzyme in a soluble environment.
AS18 MacroStab | Agrisera Universal Stabilizer preserves and stabilizes the activity of HRP or ALP conjugates and proteins and other bio-molecules. It is designed as a ready to use stabilizer, buffer, diluent and possible coat block for immunoassays.
This product can be purchased in 3 different volumes:
AS18 Macrostab-10, 10 ml.- 10ml trial size is restricted to one unit per customer -
This product is designed to stabilize coated ELISA plates and other solid phase substrates.
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