Description | Anti-MOUSE IgG3 (Gamma 3 chain) (GOAT) Antibody Rhodamine Conjugated (Min Cross Bv, Hu, and Rb Serum Proteins) |
---|---|
Host | Goat |
Conjugate | Rhodamine (TRITC) |
FP Value | 3.0 moles Rhodamine (TRITC) per mole of IgG |
Target Species | Mouse |
Reactivity | Mouse |
Clonality | Polyclonal |
Application
| WB, IF, E, IC |
Application Note | ELISA 1:2,000-1:10,000 FLISA 1:20,000-1:100,000 IF Microscopy 1:1,000-1:5,000 Western Blot 1:2,000-1:10,000 Immunochemistry 1:1,000-1:5,000 |
Physical State | Lyophilized |
Host Isotype | IgG |
Target Isotype | IgG3 |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Mouse IgG3 heavy chain |
Reconstitution Volume | 1.0 mL |
Reconstitution Buffer | Restore with deionized water (or equivalent) |
Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.01% (w/v) Sodium Azide |
Shipping Condition | Ambient |
---|---|
Purity | Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal. |
Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |