Dot blot of Lambda DNA with and without m6A, using MeRIP was performed using M6A and control RNA
Product Details Product Name Anti-N6-Methyladenosine (m6A) Rabbit Monoclonal Antibody, Clone RM362 Product Description Rabbit monoclonal to N6-Methyladenosine (m6A) Catalog No. 31-1248-00 Clone Name RM362 Product Data Sheet Anti-N6-Methyladenosine (m6A) Rabbit Monoclonal Antibody Data Sheet RM362 Specificity RM362 reacts to N6-Methyladenosine (m6A) in both RNA and DNA. No cross reactivity with non-methylated adenosine. Immunogen BSA-conjugated N6-Methyladenosine Application Dot Blot
ELISA
Methylated RNA Immunoprecipitation
Species Reactivity Human
Vertebrates Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 50 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage DotBlot: 0.5 µg/mL – 2 µg/mL;
ELISA: 0.1 µg/mL – 3 µg/mL;
MeRIP: 0.2 µg/mL – 5 µg/mL
Keywords . Product Images
anti-m6A antibody, Clone RM362. The membrane was
pre-spotted with 5, and 0.5 ng/dot of lambda DNA.
m6A-free DNA was isolated from bacteriophage
lambda grown in an E. coli host-deficient in adenine
methylase (dam-).
from NEB (E1610). Antibodies (Anti-m6A RM362
and Anti-PEG RM105) were mixed for a short time,
with Protein A beads before the addition of m6A
labelled and non- labelled RNA (in equal amounts).
Captured RNA was then washed, eluted and run
through RT-PCR. Resulting cDNA was then analyzed
using Realtime PCR with specific probes to m6A
labelled and un-labelled sequences.
Anti-N6-Methyladenosine (m6A) Rabbit Monoclonal Antibody [RM362]
![Anti-N6-Methyladenosine (m6A) Rabbit Monoclonal Antibody [RM362]](https://www.hexabiogen.com/upload/-img-lapin1.png)
Référence 31-1248-00
Conditionnement : 50ug
Marque : RevMab Biosciences
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
Rabbit monoclonal to N6-Methyladenosine (m6A)
50 µg
Vous serez peut-être également intéressé par les produits suivants :
Référence
Description
Cond.
Prix HT