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> Anti-Phospho-IGF1R/INSR-Y1165/116 & Y1189/1190 antibody

Anti-Phospho-IGF1R/INSR-Y1165/116 & Y1189/1190 antibody

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Référence NB-22-47465-50

Conditionnement : 50μL

Marque : Neo Biotech

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Téléphone : +1 850 650 7790

  • Western blot analysis of extracts of HeLa cells, using Phospho-IGF1R-Y1165/1166/INSR-Y1189/1190 polyclonal antibody at 1:1000 dilution. HeLa cells were treated by IGF-1 (50 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from HeLa cells, using Phospho-IGF1R-Y1165/1166/INSR-Y1189/1190 polyclonal antibody at 1:1000 dilution. HeLa cells were treated by IGF-1 (50 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

General Info

Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-IGF1R/INSR-Y1165/116 & Y1189/1190 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Immunogen: A synthetic phosphorylated peptide around Y1165 & Y1166 of human IGF1RIGF1R (NP_000866.1).
Immunogen Sequence: TDYYRK

Description

Background Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription, DNA-templated. Predicted to be located in nucleus.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance