Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

Anti-Phospho-IRS1-S612 antibody

Imprimer

Référence NB-22-48979-200

Conditionnement : 200μL

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Marque : Neo Biotech

Contactez votre distributeur local :

Téléphone : +1 850 650 7790

Western blot analysis of extracts of C2C12 cells, using Phospho-IRS1-S612 antibody at 1:1000 dilution. C2C12 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.
Immunohistochemistry analysis of Phospho-IRS1-S612 in paraffin-embedded rat liver using Phospho-IRS1-S612 Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry of paraffin-embedded human colon using Phospho-IRS1-S612 rabbit polyclonal antibody at dilution of 1:100 (40x lens).
Immunohistochemistry analysis of Phospho-IRS1-S612 in paraffin-embedded mouse liver using Phospho-IRS1-S612 Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry of paraffin-embedded mouse liver using Phospho-IRS1-S612 rabbit polyclonal antibody at dilution of 1:100 (40x lens).
Immunohistochemistry analysis of Phospho-IRS1-S612 in paraffin-embedded human colon using Phospho-IRS1-S612 Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry of paraffin-embedded rat liver using Phospho-IRS1-S612 rabbit polyclonal antibody at dilution of 1:100 (40x lens).
Western blot analysis of lysates from MCF7 cells using Phospho-IRS1-S612 Rabbit polyclonal antibody at 1:1000 dilution. MCF7 cells were treated by Insulin (100 nM) at 37 °C for 30 minutes after serum starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of lysates from C2C12 cells using Phospho-IRS1-S612 Rabbit polyclonal antibody at 1:1000 dilution. C2C12 cells were treated by Insulin (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

General Info

Host:	Rabbit
Applications:	WB/IHC
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-IRS1-S612 is suitable for use in Western Blot and Immunohistochemistry research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IHC-P 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	IRS1
Gene ID:	3667
Uniprot ID:	IRS1_HUMAN
Immunogen:	A synthetic phosphorylated peptide around S612 of human IRS1 (NP_005535.1).
Immunogen Sequence:	PMSPG

Description

Post Translational Modifications	Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-896 is required for GRB2-binding. Phosphorylated by ALK. Phosphorylated at Ser-270, Ser-307, Ser-636 and Ser-1101 by RPS6KB1.phosphorylation induces accelerated degradation of IRS1. Phosphorylated on tyrosine residues in response to insulin. In skeletal muscles, dephosphorylated on Tyr-612 by TNS2 under anabolic conditions.dephosphorylation results in the proteasomal degradation of IRS1. Ubiquitinated by the Cul7-RING(FBXW8) complex in a mTOR-dependent manner, leading to its degradation: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2).
Function	May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Protein Name	Insulin Receptor Substrate 1 Irs-1
Database Links	Reactome: R-HSA-109704 Reactome: R-HSA-112399 Reactome: R-HSA-112412 Reactome: R-HSA-1257604 Reactome: R-HSA-1266695 Reactome: R-HSA-198203 Reactome: R-HSA-201556 Reactome: R-HSA-2219530 Reactome: R-HSA-2428928 Reactome: R-HSA-2586552 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-74713 Reactome: R-HSA-74749 Reactome: R-HSA-9603381 Reactome: R-HSA-9725370 Reactome: R-HSA-982772
Alternative Antibody Names	Anti-Insulin Receptor Substrate 1 antibody Anti-Irs-1 antibody Anti-IRS1 antibody

Information sourced from Uniprot.org

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