Anti-Phospho-VASP-Ser157 antibody (100-180)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	VASP
Gene ID:	7408
Uniprot ID:	VASP_HUMAN
Immunogen Region:	124-173 aa
Specificity:	Phospho-VASP (S157) Polyclonal Antibody detects endogenous levels of VASP protein only when phosphorylated at S157.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human VASP around the phosphorylation site of Ser157 at the amino acid range 124-173

Post Translational Modifications	Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG/PRKG1, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation at Ser-157 by PKA is required for localization to the tight junctions in epithelial cells. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Phosphorylation at Ser-322 by AMPK also alters actin filament binding. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.
Function	Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Protein Name	Vasodilator-Stimulated Phosphoprotein Vasp
Database Links	Reactome: R-HSA-202433 Reactome: R-HSA-376176 Reactome: R-HSA-446353
Cellular Localisation	Cytoplasm Cytoskeleton Cell Junction Focal Adhesion Tight Junction Cell Projection Lamellipodium Membrane Filopodium Membrane Targeted To Stress Fibers And Focal Adhesions Through Interaction With A Number Of Proteins Including Mrl Family Members Localizes To The Plasma Membrane In Protruding Lamellipodia And Filopodial Tips Stimulation By Thrombin Or Pma Also Translocates Vasp To Focal Adhesions Localized Along The Sides Of Actin Filaments Throughout The Peripheral Cytoplasm Under Basal Conditions In Pre-Apoptotic Cells Colocalizes With Mefv In Large Specks (Pyroptosomes)
Alternative Antibody Names	Anti-Vasodilator-Stimulated Phosphoprotein antibody Anti-Vasp antibody Anti-VASP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance