Anti-PMS2 antibody (620-670) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.65-0.69 µg/µl
Dilution Range:	WB: 1:500-1:1, 000 DB: 1:20, 000-1:50, 000 ELISA: 1:20, 000-1:50, 000 IP: 1:200 IHC: 1:100
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Immunogen Region:	620-670
Immunogen:	Synthetic peptide taken within amino acid region 620-670 on human Mismatch Repair endonuclease PMS2 protein.

Background

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance