Anti-PODXL antibody (200-250) {FITC}

Clonality:	Polyclonal
Conjugation:	FITC
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.55-0.75 µg/µl
Dilution Range:	WB: 1:500 DB: 1:4, 000 ELISA: 1:4, 000 IP: 1:150 IHC: 1:50-1:100
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	PODXL
Gene ID:	5420
Uniprot ID:	PODXL_HUMAN
Immunogen Region:	200-250
Specificity:	This antibody will react with PODXL isoform 1 and will not detect PODXL isoform 2.
Immunogen:	Synthetic peptide corresponding amino acid region 200-250 on human PODXL protein.

Tissue Specificity	Glomerular epithelium cell (podocyte).
Post Translational Modifications	N- and O-linked glycosylated. Sialoglycoprotein.
Function	Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Protein Name	Podocalyxin Gctm-2 Antigen Gp200 Podocalyxin-Like Protein 1 Pc Pclp-1
Cellular Localisation	Apical Cell Membrane Cell Projection Lamellipodium Filopodium Ruffle Microvillus Membrane Raft Membrane Single-Pass Type I Membrane Protein In Single Attached Epithelial Cells Is Restricted To A Preapical Pole On The Free Plasma Membrane Whereas Other Apical And Basolateral Proteins Are Not Yet Polarized Colocalizes With Nherf2 At The Apical Plasma Membrane During Epithelial Polarization Colocalizes With Nherf1 At The Trans-Golgi Network (Transiently) And At The Apical Plasma Membrane Its Association With The Membrane Raft Is Transient Colocalizes With Actin Filaments Ezr And Nherf1 In A Punctate Pattern At The Apical Cell Surface Where Microvilli Form Colocalizes With Ezr And Nherf2 At The Apical Cell Membrane Of Glomerular Epithelium Cells Forms Granular Punctuated Pattern Forming Patches Preferentially Adopting A Polar Distribution Located On The Migrating Poles Of The Cell Or Forming Clusters Along The Terminal Ends Of Filipodia Establishing Contact With The Endothelial Cells Colocalizes With The Submembrane Actin Of Lamellipodia Particularly Associated With Ruffles Colocalizes With Vinculin At Protrusions Of Cells Colocalizes With Itgb1 Colocalizes With Pard3 Prkci Exoc5 Ocln Rab11a And Rab8a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And Luminogenesis
Alternative Antibody Names	Anti-Podocalyxin antibody Anti-Gctm-2 Antigen antibody Anti-Gp200 antibody Anti-Podocalyxin-Like Protein 1 antibody Anti-Pc antibody Anti-Pclp-1 antibody Anti-PODXL antibody Anti-PCLP antibody Anti-PCLP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance