Anti-RIPK2 antibody (410-460) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.55 µg/µl
Dilution Range:	WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:150
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	RIPK2
Gene ID:	8767
Uniprot ID:	RIPK2_HUMAN
Immunogen Region:	410-460
Immunogen:	Synthetic peptide taken within amino acid region 410-460 on human RIPK2 protein.

Tissue Specificity	Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.
Post Translational Modifications	Polyubiquitinated via both 'Lys-63'- and 'Met-1'-linked polyubiquitin following recruitment by NOD1 or NOD2, creating docking sites for downstream effectors, triggering activation of the NF-kappa-B and MAP kinases signaling. 'Lys-63'-linked polyubiquitination by XIAP is essential for NOD2 signaling and promotes recruitment of the LUBAC complex. Also polyubiquitinated with 'Lys-63'-linked chains by PELI3, BIRC2/c-IAP1 and BIRC3/c-IAP2. Ubiquitinated on Lys-209 via 'Lys-63'-linked by ITCH. Undergoes 'Lys-63'-linked deubiquitination by MYSM1 to attenuate NOD2-mediated inflammation and tissue damage. Polyubiquitinated with 'Lys-63'-linked chains in response to Shigella infection, promoting its SQSTM1/p62-dependent autophagic degradation. Undergoes 'Met-1'-linked polyubiquitination.the head-to-tail linear polyubiquitination is mediated by the LUBAC complex in response to NOD2 stimulation 'Met-1'-linked polyubiquitination. 'Lys-63'-linked polyubiquitination by XIAP is required for recruimtent of the LUBAC complex and subsequent. Linear polyubiquitination is restricted by FAM105B/otulin, probably to limit NOD2-dependent pro-inflammatory signaling activation of NF-kappa-B. Ubiquitination at Lys-503 by ZNRF4 via 'Lys-48'-linked polyubiquitination promotes RIPK2 degradation by the proteasome.ubiquitination by ZNRF4 takes place during both acute and NOD2 tolerance conditions. Autophosphorylated. Phosphorylated at Ser-176, either via autophosphorylation or by LRRK2, enhancing activity. Autophosphorylation at Tyr-474 is required for effective NOD2 signaling. Autophosphorylation is however not essential for NOD2 signaling. Phosphorylation at Tyr-381 by Src kinase CSK occurs in a ARHGEF2-dependent manner and is required for NOD2-dependent innate immune activation. Degraded via selective autophagy following interaction with IRGM. IRGM promotes NOD1/NOD2-RIPK2 RIPosome recruitment to autophagosome membranes. RIPK2 biquitinated via 'Lys-63'-linked chains is then recognized by SQSTM1/p62, leading to the SQSTM1/p62-dependent autophagic degradation of the NOD1/NOD2-RIPK2 RIPosome. (Microbial infection) Acetylation of Ser-174, Ser-176 and Ser-178 by Yersinia YopJ prevents phosphorylation and activation, thereby promoting cell death.
Function	Serine/threonine/tyrosine-protein kinase that plays an essential role in modulation of innate and adaptive immune responses. Acts as a key effector of NOD1 and NOD2 signaling pathways: upon activation by bacterial peptidoglycans, NOD1 and NOD2 oligomerize and recruit RIPK2 via CARD-CARD domains, leading to the formation of RIPK2 filaments. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3, as well as 'Met-1'-linked (linear) polyubiquitination by the LUBAC complex, becoming a scaffolding protein for downstream effectors. 'Met-1'-linked polyubiquitin chains attached to RIPK2 recruit IKBKG/NEMO, which undergoes 'Lys-63'-linked polyubiquitination in a RIPK2-dependent process. 'Lys-63'-linked polyubiquitin chains attached to RIPK2 serve as docking sites for TAB2 and TAB3 and mediate the recruitment of MAP3K7/TAK1 to IKBKG/NEMO, inducing subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. The protein kinase activity is dispensable for the NOD1 and NOD2 signaling pathways. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappa-B activation by NOD2. Also involved in adaptive immunity: plays a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. Plays a role in the inactivation of RHOA in response to NGFR signaling.
Protein Name	Receptor-Interacting Serine/Threonine-Protein Kinase 2 Card-Containing Interleukin-1 Beta-Converting Enzyme-Associated Kinase Card-Containing Il-1 Beta Ice-Kinase Rip-Like-Interacting Clarp Kinase Receptor-Interacting Protein 2 Rip-2 Tyrosine-Protein Kinase Ripk2
Database Links	Reactome: R-HSA-168638 Reactome: R-HSA-202424 Reactome: R-HSA-209543 Reactome: R-HSA-445989 Reactome: R-HSA-450302 Reactome: R-HSA-450321 Reactome: R-HSA-5689896 Reactome: R-HSA-9020702 Reactome: R-HSA-9705671
Cellular Localisation	Cytoplasm Cell Membrane Peripheral Membrane Protein Endoplasmic Reticulum Recruited To The Cell Membrane By Nod2 Following Stimulation By Bacterial Peptidoglycans
Alternative Antibody Names	Anti-Receptor-Interacting Serine/Threonine-Protein Kinase 2 antibody Anti-Card-Containing Interleukin-1 Beta-Converting Enzyme-Associated Kinase antibody Anti-Card-Containing Il-1 Beta Ice-Kinase antibody Anti-Rip-Like-Interacting Clarp Kinase antibody Anti-Receptor-Interacting Protein 2 antibody Anti-Rip-2 antibody Anti-Tyrosine-Protein Kinase Ripk2 antibody Anti-RIPK2 antibody Anti-CARDIAK antibody Anti-RICK antibody Anti-RIP2 antibody Anti-UNQ277 antibody Anti-PRO314 antibody Anti-PRO34092 antibody

Information sourced from Uniprot.org

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