Arginase 1; ARG1; liver-type arginase; type I arginase
Human gene symbol
ARG1
Entrez gene ID
383
SwissProt
P05089
Unigene
440934
Immunogen
Recombinant human ARG1 protein fragment (around aa11-97) (exact sequence is proprietary)
Verified antibody applications
IHC (FFPE) (verified), WB (verified)
Antibody target cellular localization
Cytoplasmic
Species reactivity
Human
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistology (formalin): 2-4 ug/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Western blotting 1-2 ug/mL, Optimal dilution for a specific application should be determined by user
Positive control
293Tcells. Hepatocellular Carcinoma (HCC).
Shipping condition
Room temperature
Storage Conditions
Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status
For research use only (RUO)
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
This antibody recognizes a protein of 35-38 kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibodies to Arginase 1 label hepatocytes in normal tissues and granulocytes in peripheral blood. Arginase 1 is a sensitive and specific marker for identification of hepatocellular carcinoma.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@hexabiogen.com to inquire about stock status and lead times before placing your order.
Catalog number key for antibody number 1125, Anti-Arginase 1 (ARG1/1125)
Catalog Number Key for Primary Antibody Conjugates
Antibody # prefix
Conjugation
Ex/Em (nm)
Laser line
Detection channel
Dye Features
BNC04
CF®405S
404/431
405
DAPI (microscopy), AF405
CF®405S Features
BNC88
CF®488A
490/515
488
GFP, FITC
CF®488A Features
BNC68
CF®568
562/583
532, 561
RFP, TRITC
CF®568 Features
BNC94
CF®594
593/614
561
Texas Red®
CF®594 Features
BNC40
CF®640R
642/662
633-640
Cy®5
CF®640R Features
BNC47
CF®647
650/665
633-640
Cy®5
CF®647 Features
BNCB
Biotin
N/A
N/A
N/A
BNUB
Purified
N/A
N/A
N/A
BNUM
Purified, BSA-free
N/A
N/A
N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
