CA IX Antibody (H-11) is an IgG_2a κ mouse monoclonal CA IX antibody (also designated CAIX antibody, Carbonic anhydrase IX antibody, carbonate dehydratase metalloenzyme antibody, zinc metalloenzyme antibody, cell membrane-associated carbonic anhydrase 9 antibody, transmembrane dimeric zinc metalloenzyme antibody, or gastrointestinal tract carbonic anhydrase antibody) that detects the CA IX protein of human origin by WB, IP, IF, IHC(P) and ELISA. CA IX Antibody (H-11) is available as both the non-conjugated anti-CA IX antibody form, as well as multiple conjugated forms of anti-CA IX antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme. The human CA4 gene, which maps to chromosome 17q23, encodes CA IV, a membrane-anchored isozyme that is expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression. CA IX is specifically expressed in clear-cell renal carcinomas, whereas CA XII is highly expressed in normal tissues, such as kidney, colon and pancreas. Human CA XIV is also expressed in normal tissues, such as brain, but differs from CA XII in its expression pattern.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Human carbonic anhydrase XIV (CA14): cDNA cloning, mRNA expression, and mapping to chromosome 1.  |  Fujikawa-Adachi, K., et al. 1999. Genomics. 61: 74-81. PMID: 10512682
2. Human carbonic anhydrase IV: cDNA cloning, sequence comparison, and expression in COS cell membranes.  |  Okuyama, T., et al. 1992. Proc Natl Acad Sci U S A. 89: 1315-9. PMID: 1311094
3. Carbonic anhydrase II deficiency syndrome in a Belgian family is caused by a point mutation at an invariant histidine residue (107 His----Tyr): complete structure of the normal human CA II gene.  |  Venta, PJ., et al. 1991. Am J Hum Genet. 49: 1082-90. PMID: 1928091
4. Human carbonic anhydrases and carbonic anhydrase deficiencies.  |  Sly, WS. and Hu, PY. 1995. Annu Rev Biochem. 64: 375-401. PMID: 7574487
5. Down-regulation of transmembrane carbonic anhydrases in renal cell carcinoma cell lines by wild-type von Hippel-Lindau transgenes.  |  Ivanov, SV., et al. 1998. Proc Natl Acad Sci U S A. 95: 12596-601. PMID: 9770531

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CA IX Antibody (H-11) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-CA IX Antibody (H-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.