Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> CaMKII (Alpha-Specific) Antibody

CaMKII (Alpha-Specific) Antibody

Imprimer Imprimer
Identifiez-vous

Référence ASM10037-A680-100

Conditionnement : Onrequest

Marque : Abcepta

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

 
  • ICC/IF - CaMKII (Alpha-Specific) Antibody ASM10037
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (ASM10037). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (ASM10037) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.
  • WB - CaMKII (Alpha-Specific) Antibody ASM10037
    Western Blot analysis of Rat brain membrane lysate showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (ASM10037). Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (ASM10037) at 1:1000.
  • IHC - CaMKII (Alpha-Specific) Antibody ASM10037
    Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (ASM10037). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (ASM10037) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.
  • IHC - CaMKII (Alpha-Specific) Antibody ASM10037
    Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (ASM10037). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (ASM10037) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
  • ICC/IF - CaMKII (Alpha-Specific) Antibody ASM10037
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (ASM10037). Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (ASM10037) at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP, E, RIA
Primary Accession P11798
Other Accession NP_033922.1
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat, Bovine
Clonality Monoclonal
Description Mouse Anti-Rat CaMKII (Alpha-Specific) Monoclonal IgG1
Target/Specificity Detects ~50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.
Other Names CamK2 Antibody, CamK2A Antibody, CamK2B Antibody, CamK2D Antibody, CamK2G Antibody, CAMKA Antibody
Clone Names 6G9
Immunogen Partially purified rat CaMKII
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.1 µg/ml was sufficient for detection of CamKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Cellular Localization Cytoplasm | Mitochondrion | Nucleus | Cell Junction | Synapse | Presynaptic Cell Membrane