CD20 antibody [2H7] (PE)
Référence GTX00466-08-100test
Conditionnement : 100test
Marque : Genetex
Host | Mouse |
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Clonality | Monoclonal |
Clone Name | 2H7 |
Isotype | IgG2b |
Application | FACS |
Reactivity | Human, Baboon, Chimpanzee, Cynomolgus monkey, Pig-tailed macaque, Rhesus Monkey, Squirrel monkey, Capuchin monkey |
Package | 100 test |
View product citations for antibody GTX00466-08 on CiteAb
PRODUCT
Summary
The 2H7 antibody reacts with human CD20, expressed by pre-B cells, resting and activated B cells, follicular dendritic cells and a small subset of T cells, but not on plasma cells. CD20 is a 33-37 kDa transmembrane protein which is differentially phosphorylated in resting and active B cells. The CD20 molecule plays a role in regulating B-cell activation.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application | Recommended Dilution |
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FACS | 0.06 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test |
Not tested in other applications.
Calculated MW
33 kDa. ( Note )
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.012 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human tonsillar B cells
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Phycoerythrin (PE)
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
membrane spanning 4-domains A1 , B1 , Bp35 , CD20 , CVID5 , LEU-16 , MS4A2 , S7
Cellular Localization
Cell membrane
Background
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Database
DATA IMAGES
GTX00466-08 FACS Image FACS analysis of human peripheral blood lymphocytes using GTX00466-08 CD20 antibody [2H7] (PE). |
Clone Reference
Kotani N et al. J Biol Chem 2012; 287 (44):37109-18 Fibroblast growth factor receptor 3 (FGFR3) associated with the CD20 antigen regulates the rituximab-induced proliferation inhibition in B-cell lymphoma cells.
REVIEW
Clone Reference
Kotani N et al. J Biol Chem 2012; 287 (44):37109-18 Fibroblast growth factor receptor 3 (FGFR3) associated with the CD20 antigen regulates the rituximab-induced proliferation inhibition in B-cell lymphoma cells.
SDS | |
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PBS.pdf | |
Sodium Azide.pdf |