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> CD40L / CD154 antibody [5C8] (APC)

CD40L / CD154 antibody [5C8] (APC)

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Identifiez-vous

Référence GTX01484-07-100test

Conditionnement : 100test

Marque : Genetex

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Anti-CD40L / CD154 antibody [5C8] (APC) used in Flow cytometry (FACS). GTX01484-07

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5C8

Isotype

 IgG2a

Application

 FACS

Reactivity

 Human, Cynomolgus monkey, Rhesus Monkey
Package
100 test
Datasheet File
View product citations for antibody GTX01484-07 on CiteAb
PRODUCT

Summary

The 5C8 antibody reacts with human CD154 which is also known as CD40L (CD40 ligand). CD154 is a 39kDa member of the TNF-α superfamily and is predominantly expressed on activated T cells but is also expressed on NK cells, mast cells, basophils, and eosinophils. CD154 binds to CD40, a molecule that is expressed on antigen presenting cells including B cells, monocytes/macrophages, and dendritic cells. The interaction of CD154 and CD40 regulates the activation of antigen presenting cells and is critical for B cell costimulation. The 5C8 antibody has been reported to block CD40L-CD40 interactions.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD40 ligand , CD154 , CD40L , CD40LG , HIGM1 , IGM , IMD3 , TBAM , TNFSF5 , TRAP , gp39 , hCD40L

Cellular Localization

Cell membrane,Secreted

Background

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-CD40L / CD154 antibody [5C8] (APC) used in Flow cytometry (FACS). GTX01484-07

GTX01484-07 FACS Image

FACS analysis of human PBMCs (with PMA and Ionomycin stimulation for 5 hrs) using GTX01484-07 CD40L / CD154 antibody [5C8] (APC).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)

Clone Reference

2018; Candida-Reactive T Cells for the Diagnosis of Invasive Candida Infection-A Prospective Pilot Study.
2017; Functional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis.
2016; Highly Predictive Model for a Protective Immune Response to the A(H1N1)pdm2009 Influenza Strain after Seasonal Vaccination.
2015; Analysis of the functional WT1-specific T-cell repertoire in healthy donors reveals a discrepancy between CD4(+) and CD8(+) memory formation.
2015; Effects of aging on human leukocytes (part II): immunophenotyping of adaptive immune B and T cell subsets.

REVIEW

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Clone Reference
2018; Candida-Reactive T Cells for the Diagnosis of Invasive Candida Infection-A Prospective Pilot Study.
2017; Functional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis.
2016; Highly Predictive Model for a Protective Immune Response to the A(H1N1)pdm2009 Influenza Strain after Seasonal Vaccination.
2015; Analysis of the functional WT1-specific T-cell repertoire in healthy donors reveals a discrepancy between CD4(+) and CD8(+) memory formation.
2015; Effects of aging on human leukocytes (part II): immunophenotyping of adaptive immune B and T cell subsets.
SDS
Sodium Azide.pdf