Cholesterol

Biological Activity

Cholesterol is a major sterol in mammals, accounting for 20-25% of the structural components of the plasma membrane, and plays an important role in characterizing membrane fluidity and permeability, as well as the function of transporter and signaling proteins. In addition, Cholesterol is an endogenous estrogen-related receptor alpha (ERRα) agonist.

Chemical Information

Molecular Weight	386.65
Formula	C27H46O
CAS Number	57-88-5
Solubility (25°C)	Ethanol 8 mg/mL
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] David S Schade, et al. Cholesterol Review: A Metabolically Important Molecule

[2] Jie Luo, et al. Mechanisms and regulation of cholesterol homeostasis

[3] Vinay Narwal, et al. Cholesterol biosensors: A review

[4] Victor A Cortes, et al. Physiological and pathological implications of cholesterol

[5] H J Weis. Cholesterol metabolism