CPB1 (Carboxypeptidase B1, Tissue, CPB, PCPB, PASP, Pancreas-specific protein)

Référence 362659-200ul

Conditionnement : 200ul

Marque : US Biological

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Host
rabbit

Isotype
IgG

Grade
Affinity Purified

Applications
E IC IHC WB

Shipping Temp
Blue Ice

Storage Temp
-20°C

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.
The deduced 416aa preproenzyme (EC 3.4.17.2) has a 15aa signal peptide and a 95aa activation peptide. It shares 76% and 84% sequence identity with the rat and bovine homologs, respectively. DNA blot analysis indicated that the PCPB mRNA is transcribed from a single locus. Northern blot analysis demonstrated pancreas-specific expression.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:5000
Western Blot: 1:50-400
Immunocytochemistry: 1:50-500
Immunohistochemistry (frozen): 1:50-500
Immunohistochemistry (paraffin): 1:10-100
Optimal dilutions to be determined by the researcher.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ATGHSYEKYN KWETIEAWTQ QVATENPALI SRSVIGTTFE GRAIYLLKVG KAGQNKPAIF MDCGFHAREW ISPAFCQWFV REAVRTYGRE IQVTELLDKL DFYVLPVLNI DGYIYTWTKS RFWRKTRSTH TGSSCIGTDP NRNFDAGWCE IGASRNPCDE TYCGPAAESE KETKALADFI RNKLSSIKAY LTIHSYSQMM IYPYSYAYKL GENNAELNAL AKATVKELAS LHGTKYTYGP GATTIYPAAG GSDDWAYDQG IRYSFTFELR DTGRYGFLLP ESQIRATCEE TFLAIKYVAS YVLEHLY

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Type
Pab

Isotype
IgG

Host
rabbit

Source
human

Concentration
~0.5mg/ml

Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Purity
Purified by immunoaffinity chromatography.

Immunogen
Recombinant protein corresponding to Ala111-Tyr417 of human CPB1 expressed in E. coli.

Specificity
Recognizes human CPB1.


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.