DFHBI [1241390-29-3]
Référence HY-110250-5mg
Conditionnement : 5mg
Marque : MedChemExpress
Please arrange your schedule properly.
- JP - 日本語
- EN - English
- FR - Français
- DE - Deutsch
- ES - Español
- KR - 한국어
- Antibody-drug Conjugate/ADC Related
- Apoptosis
- Autophagy
- Cell Cycle/DNA Damage
- Cytoskeleton
- Epigenetics
- GPCR/G Protein
- Immunology/Inflammation
- JAK/STAT Signaling
- MAPK/ERK Pathway
- Membrane Transporter/Ion Channel
- Metabolic Enzyme/Protease
- Neuronal Signaling
- NF-κB
- PI3K/Akt/mTOR
- PROTAC
- Protein Tyrosine Kinase/RTK
- Stem Cell/Wnt
- TGF-beta/Smad
- Vitamin D Related/Nuclear Receptor
- Others
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
- Protocol 1
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.43 mg/mL (5.67 mM); Clear solution
This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution. - Protocol 2
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.43 mg/mL (5.67 mM); Clear solution
This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear. - Protocol 3
Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.43 mg/mL (5.67 mM); Clear solution
This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 900 μL Corn oil, and mix evenly.
Dosage
mg/kgAnimal weight
(per animal)
Dosing volume
(per animal)
Number of animals
*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)
-
[1]. Song W, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136(4):1198-201. [Content Brief]
[2]. Strack RL, et al. Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. Nat Protoc. 2014 Jan;9(1):146-55. [Content Brief]
-
[1]. Song W, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136(4):1198-201.
[2]. Strack RL, et al. Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. Nat Protoc. 2014 Jan;9(1):146-55.
* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
---|
DMSO | 1 mM | 3.9648 mL | 19.8240 mL | 39.6479 mL | 99.1198 mL |
5 mM | 0.7930 mL | 3.9648 mL | 7.9296 mL | 19.8240 mL | |
10 mM | 0.3965 mL | 1.9824 mL | 3.9648 mL | 9.9120 mL | |
15 mM | 0.2643 mL | 1.3216 mL | 2.6432 mL | 6.6080 mL | |
20 mM | 0.1982 mL | 0.9912 mL | 1.9824 mL | 4.9560 mL | |
25 mM | 0.1586 mL | 0.7930 mL | 1.5859 mL | 3.9648 mL | |
30 mM | 0.1322 mL | 0.6608 mL | 1.3216 mL | 3.3040 mL | |
40 mM | 0.0991 mL | 0.4956 mL | 0.9912 mL | 2.4780 mL | |
50 mM | 0.0793 mL | 0.3965 mL | 0.7930 mL | 1.9824 mL | |
60 mM | 0.0661 mL | 0.3304 mL | 0.6608 mL | 1.6520 mL | |
80 mM | 0.0496 mL | 0.2478 mL | 0.4956 mL | 1.2390 mL | |
100 mM | 0.0396 mL | 0.1982 mL | 0.3965 mL | 0.9912 mL |
- Others
- Fluorescent Dye
-
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
DFHBI1241390-29-3Fluorescent DyeInhibitorinhibitorinhibit
Vos produits récemment consultés:
Demande en ligne
Your information is safe with us. * Required Fields.
Bulk Inquiry
Inquiry Information
- Nom du produit:
- DFHBI
- Cat. No.:
- HY-110250
- Quantité:
- MCE Japan Authorized Agent: