EGTA Buffer 0.5M, pH 8.0, DNase and RNase Free

Référence 40121266-1

Conditionnement : 50mL

Marque : BioWorld

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

Description

EGTA [Ethyleneglycol bis (2-Aminoethyl ether)-N,N,N',N' tetraacetic acid] is of Molecular Biology grade, and is suitable for all molecular biology applications. Nuclease and protease free, EGTA is used to chelate CA2+ ions in the presence of Mg2+. EGTA chelates these two ions at a 1:1 ratio. EGTA is also used as a calibration standard for calcium ion concentration, with detection accurate to 10 �M. It can also be used as an anticoagulant at a concentration of 1 g per 100 mL of blood.

EGTA is soluble in 1M NaOH (110 mg/mL). As a saturated solution at room temperature, EGTA concentration is found to be 2 mM at a pH of 2.72.

References:

Greenberg S, el Khoury J, Kaplan E, Silverstein SC 1991. A fluorescence technique to distinguish attached from ingested erythrocytes and zymosan particles in phagocytosing macrophages. J Immunol Methods 139:115-122.

Bers DM, Patton CW, Nuccitelli R (1994) A practical guide to the preparation of Ca2+ buffers. Methods Cell Biol 40:3-29.

Ouanounou A, Zhang L, Tymianski M, Charlton MP, Wallace MC, Carlen PL (1996) Accumulation and extrusion of permeant Ca2+ chelators in attenuation of synaptic transmission at hippocampal CA1 neurons. Neuroscience (1996) 75:99-109.

Properties

Shelf life

3 years

Storage Temperature

RT

ECCN #

EAR88

Hazmat Ship

Non-hazardous

Concentration

0.5M

Appearance color

Clear

Appearance form

Liquid

pH value

8.0

Vous serez peut-être également intéressé par les produits suivants :



Référence
Description
Cond.
Prix HT
30630216-2
 25mL