Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> ERAB antibody [10B4]

ERAB antibody [10B4]

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Identifiez-vous

Référence GTX84346-100ul

Conditionnement : 100ul

Marque : Genetex

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Téléphone : +1 850 650 7790

Anti-ERAB antibody [10B4] used in Flow cytometry (FACS). GTX84346
Anti-ERAB antibody [10B4] used in Flow cytometry (FACS). GTX84346
Anti-ERAB antibody [10B4] used in IHC (Paraffin sections) (IHC-P). GTX84346
Anti-ERAB antibody [10B4] used in Western Blot (WB). GTX84346
Anti-ERAB antibody [10B4] used in Western Blot (WB). GTX84346
Anti-ERAB antibody [10B4] used in IHC (Paraffin sections) (IHC-P). GTX84346
Anti-ERAB antibody [10B4] used in IHC (Paraffin sections) (IHC-P). GTX84346
Anti-ERAB antibody [10B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84346
Anti-ERAB antibody [10B4] used in IHC (Paraffin sections) (IHC-P). GTX84346
Anti-ERAB antibody [10B4] used in IHC (Paraffin sections) (IHC-P). GTX84346

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10B4

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl
Datasheet File
View product citations for antibody GTX84346 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human HSD17B10(NP_004484) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxysteroid 17-beta dehydrogenase 10 , 17b-HSD10 , ABAD , CAMR , DUPXp11.22 , ERAB , HADH2 , HCD2 , HSD10MD , MHBD , MRPP2 , MRX17 , MRX31 , MRXS10 , SCHAD , SDR5C1

Cellular Localization

Mitochondrion

Background

Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD).

Database

DATA IMAGES

Anti-ERAB antibody [10B4] used in Flow cytometry (FACS). GTX84346

GTX84346 FACS Image

FACS analysis of HeLa cells using GTX84346 ERAB antibody [10B4].
Red : Primary antibody
Blue : Negative control antibody

Anti-ERAB antibody [10B4] used in Flow cytometry (FACS). GTX84346

GTX84346 FACS Image

FACS analysis of Jurkat cells using GTX84346 ERAB antibody [10B4].
Red : Primary antibody
Blue : Negative control antibody

Anti-ERAB antibody [10B4] used in IHC (Paraffin sections) (IHC-P). GTX84346

GTX84346 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84346 ERAB antibody [10B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ERAB antibody [10B4] used in Western Blot (WB). GTX84346

GTX84346 WB Image

WB analysis of various cell lines using GTX84346 ERAB antibody [10B4].
Loading : 35 ug per lane

Anti-ERAB antibody [10B4] used in Western Blot (WB). GTX84346

GTX84346 WB Image

WB analysis of HEK293T cells transfected with ERAB plasmid (Right) or empty vector (Left) for 48 hrs using GTX84346 ERAB antibody [10B4].
Loading : 5 ug per lane

Anti-ERAB antibody [10B4] used in IHC (Paraffin sections) (IHC-P). GTX84346

GTX84346 IHC-P Image

IHC-P analysis of kidney tissue using GTX84346 ERAB antibody [10B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ERAB antibody [10B4] used in IHC (Paraffin sections) (IHC-P). GTX84346

GTX84346 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84346 ERAB antibody [10B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ERAB antibody [10B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84346

GTX84346 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with ERAB plasmid using GTX84346 ERAB antibody [10B4].

Anti-ERAB antibody [10B4] used in IHC (Paraffin sections) (IHC-P). GTX84346

GTX84346 IHC-P Image

IHC-P analysis of liver tissue using GTX84346 ERAB antibody [10B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ERAB antibody [10B4] used in IHC (Paraffin sections) (IHC-P). GTX84346

GTX84346 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX84346 ERAB antibody [10B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf