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> ERp57 Antibody

ERp57 Antibody

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Référence ASM10174-STR-100

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  • ICC/IF - ERp57 Antibody ASM10174
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ERp57 Monoclonal Antibody, Clone 4F9 (ASM10174). Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ERp57 Monoclonal Antibody (ASM10174) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic reticulum, Melanosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ERp57 Antibody (D) Composite.
  • WB - ERp57 Antibody ASM10174
    Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of 57 kDa Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone 4F9 (ASM10174). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Load: 15 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (ASM10174) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 57 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P30101
Other Accession NP_005304.3
Host Mouse
Isotype IgG2b
Reactivity Human
Clonality Monoclonal
Description Mouse Anti-Human ERp57 Monoclonal IgG2b
Target/Specificity Detects ~57 kDa protein.
Other Names ERp60 Antibody, ERp61 Antibody, Grp57 Antibody, Grp58 Antibody, P58 Antibody, PDIA3 Antibody, PI PLC Antibody, 58 kDa glucose regulated protein Antibody, 58 kDa glucose-regulated protein Antibody, 58 kDa microsomal protein Antibody, Disulfide isomerase ER 60 Antibody, Disulfide isomerase ER-60 Antibody, Endoplasmic reticulum resident protein 57 Antibody, Endoplasmic reticulum resident protein 60 Antibody, ER p57 Antibody, ER protein 57 Antibody, ER protein 60 Antibody, ERp 57 Antibody, ERp57 Antibody, Glucose Regulated Protein 58 Kd Antibody, GRP 57 Antibody, GRP 58 Antibody, GRP57 Antibody, HsT17083 Antibody, p58 Antibody, PDIA 3 Antibody, PDIA3 Antibody, PDIA3_HUMAN Antibody, Phospholipase C alpha Antibody, PI PLC Antibody, Protein disulfide isomerase A3 Antibody, Protein disulfide isomerase family A member 3 Antibody, Protein disulfide-isomerase A3 Antibody
Clone Names 4F9
Immunogen Human recombinant ERp57 (Grp58).
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SMC-266 was sufficient for detection of ERp57 in 15 µg of HeLa by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
Cellular Localization Endoplasmic Reticulum | Endoplasmic Reticulum Lumen | Melanosome