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> F(ab')2 Human lambda antibody (Alkaline Phosphatase)

F(ab')2 Human lambda antibody (Alkaline Phosphatase)

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Référence orb348193-500ug

Conditionnement : 500ug

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    F(ab')2 Human lambda antibody (Alkaline Phosphatase)

    F(ab')2 Human lambda antibody (Alkaline Phosphatase)

    Catalog Number: orb348193

    Catalog Numberorb348193
    CategoryAntibodies
    DescriptionF(ab')2 Human lambda antibody (Alkaline Phosphatase)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityHuman
    IsotypeIgG F(ab')2
    ImmunogenHuman lambda light chain
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
    Form/AppearanceLiquid (sterile filtered)
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
    ConjugationAP
    StorageStore vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesGoat F(ab')2 Anti-Human λ (lambda chain) Antibody
    NoteFor research use only
    Application notesThis product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
    Expiration Date12 months from date of receipt.